Suppression of LIM Kinase 1 and LIM Kinase 2 Limits Glioblastoma Invasion

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The N-terminal LIM domain negatively regulates the kinase activity of LIM-kinase 1.

LIM-kinase 1 (LIMK1, where LIM is an acronym of the three gene products Lin-11, Isl-1 and Mec-3) is a serine/threonine kinase that phosphorylates cofilin and regulates actin cytoskeletal reorganization. LIMK1 contains two LIM domains and a PDZ (an acronym of the three proteins PSD-95, Dlg and ZO-1) domain in the N-terminal half and a kinase domain in the C-terminal half. In this study we examin...

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Downregulation of LIM kinase 1 suppresses ocular inflammation and fibrosis

PURPOSE The purpose of this study was to determine if downregulation of LIM kinase 1 (LIMK1) by genetic deletion or direct application of LIMK1-targeted siRNA could suppress TGF-beta mediated ocular inflammation and fibrosis. METHODS LIMK1 specific siRNAs designed from the human sequence were transfected into human corneal fibroblasts in culture. Immunofluorescence and immunoblotting were per...

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ژورنال

عنوان ژورنال: Cancer Research

سال: 2019

ISSN: 0008-5472,1538-7445

DOI: 10.1158/0008-5472.can-19-1237